The Centrifugal Microfluidics Conference Special Issue is a curated collection of research papers selected from the inaugural International Conference on Centrifugal Microfluidics, held in April 2024 in Mexico City. This special issue, published in Microsystems & Nanoengineering, encompasses cutting-edge studies and advancements in centrifugal microfluidics, also known as Lab-on-a-Disc technology. The featured papers delve into the transformative potential of this technology across various sectors, addressing challenges and exploring applications in medical diagnostics, water treatment, and innovative microfluidic methodologies.