The minimum tracking interval distance (TID) of trains is a crucial operational element in the scheduling of Medium-Speed Maglev (MSM) train timetables. In the scenario of mixed passenger and freight operations, the differences in traction performance between MSM passenger trains and freight trains result in challenges for calculating the tracking interval. Therefore, this paper proposes a method for calculating the minimum TID by considering the different parameters and operational strategies of passenger and freight trains in mixed transport scenarios. First, this paper constructs a simulation model for the operation of MSM trains. Next, based on safety braking principles and the MSM train operation simulation approach, a dynamic tracking simulation model for MSM trains is established. Finally, this paper simulates the operating speed curves of passenger and freight trains sequentially. The minimum dispatch interval distance is calculated by evaluating the safety constraints during the operation of MSM trains and employing an iterative optimization method. To validate the proposed method, simulation tests were conducted using constructed MSM lines and data from passenger and freight trains. Two cases of mutual tracking between passenger and freight trains were specifically used to verify the method for calculating the TID of MSM trains in the scenario of mixed passenger and freight operations. The simulation results indicate that the method can calculate the minimum TID for passenger and freight trains in mixed operations.