The intensifying competition within the global textile market has presented significant challenges for research scientists and industrial manufacturers alike. The exponential growth of technical textiles and their diverse applications has fostered a plethora of opportunities for the implementation of innovative finishing techniques. Given the ability of microorganisms and bloodborne pathogens to transmit an array of diseases, the development of a textile fabric possessing both antimicrobial and blood repellent properties has become a pressing need. Through the creation of such a product, cross-infections arising from the utilization of bed linens and wound dressings that encounter wound blood can be effectively mitigated. The antimicrobial efficacy of a methanol extract derived from the clove herb was evaluated using a standardized agar diffusion technique. The clove extract was subsequently assessed against Escherichia coli, a Gram-negative bacterial species. The findings revealed noteworthy antimicrobial activity against the polyester/cotton blend fabric. Citric acid, employed at varying ratios of metal to liquor to reductant (1,50, 1:70, and 1:90), was utilized for the post-treatment of the clove extract-treated fabric. The union-dyed fabric exhibited pronounced antimicrobial activity, whereas the reactive-dyed fabric demonstrated a comparatively smaller degree of antimicrobial efficacy. Blood repellency was evaluated across distinct time intervals (5, 10, and 30 minutes). The finished fabric, upon treatment with the blood repellent finish, displayed exceptional performance. Notably, various physical properties of the finished fabric, including tensile strength, bending length, and crease recovery, were demonstrably enhanced.