The characterization of secondary metabolites extracted from acacia gums (AGs) is crucial for understanding their chemical composition and potential benefits. Various analytical techniques, such as chromatography and spectroscopy, have been used to determine the structures and properties of these compounds. Identifying and characterizing secondary metabolites from AGs can shed light on their chemical composition and biological activities, thereby providing valuable insights into their potential applications in medicine, agriculture, and industry. This chapter provides a comprehensive overview of the characterization of secondary metabolites extracted from AGs and sheds light on their chemical composition.