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Stochastic optical reconstruction microscopy or STORM is a technique to achieve optical super-resolution. STORM implementation in a standard optical microscope with contemporary imaging methods can be complex and adds a fair amount in terms of expenses. We present a low-cost but effective approach for implementing STORM by developing a modular optical microscope that is affordable and flexible in many ways from transportation to the utilization of industry-grade and economical components with open-source software for image acquisition and analysis.

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A Cost-Effective Implementation of Optical Super-Resolution Microscopy with STORM

  • 会议论文

  • 发表日期：

    2025年

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  • Anupam Bharadwaj,
  • Pranjal Choudhury,
  • Ranjan Kalita,
  • Amit Sharma,
  • Amalesh Kumar,
  • Bithiah Grace Jaganathan,
  • Sunil Kumar,
  • Jonathan Lightley,
  • Edwin Garcia,
  • P. M. W. French,
  • Bosanta Ranjan Boruah

摘要

Stochastic optical reconstruction microscopy or STORM is a technique to achieve optical super-resolution. STORM implementation in a standard optical microscope with contemporary imaging methods can be complex and adds a fair amount in terms of expenses. We present a low-cost but effective approach for implementing STORM by developing a modular optical microscope that is affordable and flexible in many ways from transportation to the utilization of industry-grade and economical components with open-source software for image acquisition and analysis.