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Birefringence of biological samples is associated with anisotropic refractive index variation which depends on the polarisation and propagation direction of the illumination light. Here, we propose polarisation-sensitive non-interferometric quantitative phase microscopy to extract phase maps along different polarisation axes. Transport of intensity equation is used to reconstruct the phase of biological tissue by means of exploiting polarisation-dependent variation of phase value of the biological tissue. Experimental results of cancer tissue are presented in this work.

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Polarisation-Dependent Quantitative Phase Imaging Using Transport of Intensity Equation

  • 会议论文

  • 发表日期：

    2025年

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  • Himanshu Joshi,
  • Sunil Bhatt,
  • Ankit Butola,
  • Pramila Thapa,
  • Deepika Mishra,
  • Dalip Singh Mehta

摘要

Birefringence of biological samples is associated with anisotropic refractive index variation which depends on the polarisation and propagation direction of the illumination light. Here, we propose polarisation-sensitive non-interferometric quantitative phase microscopy to extract phase maps along different polarisation axes. Transport of intensity equation is used to reconstruct the phase of biological tissue by means of exploiting polarisation-dependent variation of phase value of the biological tissue. Experimental results of cancer tissue are presented in this work.