Bioprinting has arisen as a promising technique in tissue engineering, presenting the opportunity to fabricate tissue constructs that closely emulate physiological function. However, a significant obstacle in three-dimensional bioprinting lies in the development of suitable bioinks. Silk fibroin (SF), known for its high biocompatibility, biodegradability, and machinability has garnered attention as a potential bioink candidate. Integrating silk into bioink formulations, alongside other natural and synthetic polymers, enhances viscosity, though challenges exist in optimizing gelation properties for effective bioprinting. Functionalization strategies are essential to render SF compatible with bioprinting techniques, ensuring suitable characteristics and mechanical properties printing. This book section presents an in-depth exploration of silk-based bioinks, offering insights into their diverse formulations, properties and functionalization strategies. Emphasizing their versatility, and showcases the potential of silk-based bioinks in various bioprinting applications across cartilage, skin, bone, and nerve tissue engineering. Through a comprehensive analysis, the review underscores the critical role of continued innovation and collaboration in unlocking the complete therapeutic capabilities of silk-based bioinks in regenerative medicine and tissue engineering.