The additive manufacturing of silk has garnered significant research interest because of its distinct attributes, such as biodegradability, biocompatibility, and tendency to be combined with other materials. These materials are extensively utilized in many different industries, including medicine, biomedical engineering, support for medical scaffolds, delivering medication, food processing, detecting biological substances, and creating bioelectrical devices. The continuous protein fiber is known as silk; certain insect larvae produce silk cocoons. Silk fibroin, a structural protein, can be handled through several techniques to create a range of material formats, encompassing hydrogels, inks, resins, and fibers. These diverse material formats can then be employed in different 3D printing techniques to fabricate complex structures and designs. 3D printing techniques are indeed categorized based on both material and technical aspects. This book chapter provides a better understanding of the 3D printing techniques for silk-based material, various fabrication approaches to develop silk-based material, applications, and challenges faced during the 3D printing of silk-based material.