Point-of-care testing offers rapid, simple, and portable diagnostic tests, enabling healthcare professionals to make timely treatment decisions. These tests represent a valuable clinical strategy in different situations, particularly in remote and resource-limited settings, where access to traditional laboratories is limited. Point-of-care testing plays a vital role in diagnosing a wide range of conditions, from infectious diseases to chronic illnesses, facilitating early intervention and improving patient outcomes. With technological advancements, point-of-care testing continues to evolve, providing accurate and reliable results within minutes, thus enhancing healthcare delivery and patient care worldwide. Consequently, exploring different materials and analytical techniques for new device proposals is an important area of study. Electrochemical biosensors offer improved sensitivity and selectivity compared to conventional immunoassay tests, making them promising candidates for point-of-care testing. Graphene, a nanomaterial, is especially noteworthy for its good electron transfer kinetics and biocompatibility, enhancing the analytical performance of the biosensors. This chapter aims to provide a concise review of recent developments in electrochemical biosensors, including immunosensors, genosensors, and aptasensors, constructed using graphene and its derivates or utilizing these materials as modifying agents for electrode surfaces, with a focus on their potential for point-of-care testing. The discussion will cover the main aspects of the construction of these biosensors, highlighting their analytical characteristics and applications.