The disciplines of veterinary and animal sciences make significant contributions to the fields of comparative medicine and pathology, food safety and public health, animal health and welfare, wildlife and fisheries conservation, epidemiology and surveillance of infectious disease, discovery and design of drugs, and vaccine development, respectively. In order to gain significant benefits from the “One Health Approach,” which includes humans, animals, and environmental health, bioinformatics plays an important role in providing the interdisciplinary framework for collaborative veterinary research. A good example of how vet informatics is used in veterinary and animal sciences is the use of databases, resources and computational tools. The goal, circumstances, and restrictions for the data must be understood by the user in order to make the best use of animal disease stored data. The potential use of the databases has many objectives, including food safety, animal welfare and economics of animal health. Initially, many databases and tools were created for a variety of uses, such as calculating cattle breeding values or logging particular infections to show that a population is disease-free. Over many years, the databases and tools have been further developed in accordance with the unique conditions, limitations, and requirements of the particular species. Evidently, it meant that all the disease data have rationality in giving disease severity information in terms of welfare or in terms of loss of production or in terms of food safety. The aim of this chapter is to highlight the available database resources and computational tools as well as their application in livestock research for the benefit of the veterinary science community.