In tissue cultures, surface sterilization is one of the most crucial concerns. The subsequent steps cannot be completed without success in the surface sterilization phase. Because of this, the most important factor affecting a culture’s efficacy is the surface sterilization of plant parts (explants). When surface sterilizing explants, sterilization agents like sodium hypochlorite, ethanol, calcium hypochlorite, hydrogen peroxide, mercury chloride, different surfactants (such as Tween 20), and plant protection combinations are commonly utilized. These sterilant are often poisonous; they discolour objects and prevent growth and development in vitro. Nowadays, nanotechnology is applied successfully in numerous fields. In plant tissue culture, it is evident that silver nanoparticles (Ag NPs) are widely employed in antibacterial and antiseptic applications. One technique for producing Ag NPs other than physical and chemical synthesis is biosynthesis. When it comes to all known forms of microorganisms in the literature, silver ions are the most efficient antimicrobial and antibacterial compounds. They are not only better than other antimicrobial agents, but they are also safe for the environment and human health.