The transport of nucleic acids to host sites must be successful in creating gene therapy techniques. Due to their capacity to shield nucleic acids from deterioration and improve their cellular absorption and bioavailability, PEGylated nanocarrier systems have demonstrated significant potential as delivery technologies. This manuscript outlines creating and evaluating a PEGylated nanocarrier system for transporting nucleic acids. The nanocarrier comprised a lipid-based core for encasing nucleic acids and a polyethylene glycol (PEG) coating to increase stability and decrease immunogenicity. The nanocarrier may effectively encapsulate and shield the nucleic acids from nuclease deterioration. Moreover, it displayed in vitro minimal cytotoxicity and good cellular uptake and transfection efficiency. In vivo tests revealed that considerable gene activation in the liver was caused by the nanocarrier’s ability to effectively carry nucleic acids to target cells in a mouse model.