A measure of a waterbody’s water quality and, consequently, its ability to support aquatic life, is the dissolved oxygen (DO) concentration in the water. A hydraulic jump is caused by a swift or abrupt conversion of the flow commencing from supercritical form to subcritical. It can be found at the bottoms of spillways, in channels, canals beneath sluiceways, or when a slope suddenly becomes flatter after steepness. It causes abrupt rises in water level, surface-roller formation, and energy dissipation. Using these as a potent natural blender for oxygen transport can elevate DO levels in the water bodies, causing turbulence. The primary cause of this oxygen transmission is the entrainment of air into the stream through numerous air foams, which aids the air–water transfer. In this investigation, hydraulic jumps were developed in a rectangular laboratory channel, performing a series of experiments, to observe jump parameters and aeration efficiency varying discharge and bed slope. Hydraulic jump, herein, is formed by adjusting a tailgate and experiments are done at five different discharges and two different bed slopes. During experimentation, the inlet Froude number (Fr1) before the jump ranges between 2.18 to 7.95. Between the hydraulic jump controlling parameters with the rate of energy dissipation and aeration efficiency high correlations are observed. Throughout the series of experiments, it is found that the aeration efficiency varies from 0.1096 to 0.3333. Results conclude that the hydraulic jump effectively dissipates energy and transfers oxygen. This research has applications in estimating the efficiency of hydraulic jump aeration.