Agriculture production suffers substantially due to insect pest infestation. In the majority of cases, pest management programs involved the use of harmful chemical pesticides, which has serious human health and environmental implications. On the contrary, the use of biopesticides over several years is proven as safe alternative method which supports agriculture in a sustainable manner. Prevalent biopesticides include the bioactive compounds which are majorly expressed in microorganisms such as algae, fungi, bacteria and some plant species. Since past few decades, the double-stranded RNA (dsRNA)-based RNA interference (RNAi) technology has become the upcoming revolutionized approach to deliver insect-resistant plant varieties which acts as biopesticides. RNAi works on the principle of sequence-specific gene silencing. In this phenomenon, dsRNA acts as the key triggering molecule that induces the formation of RNA-induced silencing complex (RISC) and regulates the expression of specific mRNA targets based on complementarity. RNAi has been shown to work effectively without involving the expression of foreign protein in the plant system and therefore reduces the selection pressure on notorious insect-pest to evolve. These properties make it selective and topically applicable approach for crop protection. In this chapter, the basic RNAi technology and its application in insect-pest control as biopesticides are discussed. Moreover, the advantages and risk-associated with this technology are also highlighted. The increasing understanding of RNAi technology and its cost-effective applications, provide a potential method to sustainable agriculture.