Basic methods for NMR analysis of RNA are presented for researchers who recently joined or want to join this field. This chapter includes the design of RNA sequences, sample preparation, NMR measurements, and analysis of exchangeable and non-exchangeable proton signals as well as structure calculation. It is usually required to design shorter RNA fragments to be analyzed by NMR. RNA samples are prepared by in vitro transcription or chemical synthesis. In addition, the enzymatic ligation method and artificial base pair systems can be used. Because proton signals of RNA are usually overlapped, stable-isotopic labeling is required to separate the overlapped signals. Site-specific 10% 13C/15N-labeled RNAs are useful to identify signals for the labeled residue. Because RNAs are usually elongated molecules, long-range constraints such as residual dipolar couplings are useful for obtaining information on the global conformation of RNAs. Pf1 filamentous phage has been generally used for the alignment of RNAs. Some examples of Interaction analysis and structure screening are also shown. Furthermore, analysis for longer RNAs and in-cell NMR are also introduced.