Start-ups are founded to develop some unique offerings to the market, which are crucial for any entrepreneurial economy. They are adept at bringing economic and social change through economic recovery and responsible growth. However, to successfully influence the rate of these new enterprises, an attempt should be made to understand how these start-ups’ ecosystem factors impact a particular economy. Start-ups’ competitive edge gain from many external factors relating to academia or university tie-ups, industry associations, government bodies, and civil society, which constitute the core pillars of the quadruple helix approach. Hence this study fulfills the gap by bringing the conceptual framework of the quadruple helix model: into context to India and then by understanding the impact of these enabling factors on the pillars of the ecosystem through participatory action research performed in the ecosystem of Gujarat. The implications drawn are similar to what was proposed by the participant in the Action Seminar and the five-year report on state start-up ranking framework.