The global One Health index (GOHI) study provides an extensive assessment of health interrelations among humans, animals, and the environment. Globally, the average GOHI score is 54.82, signaling substantial room for improvement. The study reveals marked disparities in GOHI scores across various regions and countries, suggesting uneven performances in managing health issues in the interface of human, animal and ecosystem. In examining specific nations, Oman’s commendable efforts in animal and environmental health contrast its human health performance, while Singapore excels in human health but lags in environmental health. Among the GOHI-Governance indicators, consensus-oriented methods score highest, but the formation of specialized One Health associations is lacking. The GOHI-Zoonoses score is notable, but the route of transmission indicator highlights weaknesses in managing zoonotic disease pathways. These findings underscore the need for robust international cooperation, enhanced data sharing mechanisms, and the translation of One Health research into actionable policies. The study’s limitations include potential data collection biases and the need for further validation of the GOHI framework.