The global One Health index (GOHI) assesses One Health approaches across human, animal, and environmental sectors. Significant disparities in GOHI performance exist worldwide, with the United States achieving the highest score (70.61) and Guinea-Bissau the lowest (39.03). Overall, the global average score stands at 54.82, suggesting considerable room for improvement in One Health practice. Notably, income levels correlate with One Health performance, with high-income regions like North America, Europe, and East Asia achieving better scores than low-income regions such as sub-Saharan Africa. Despite variations, no country reached the optimal score in all indicators of core drivers index. The study further emphasizes the importance of international cooperation, especially between high- and low-income countries, to enhance One Health outcomes. The pandemic has highlighted the significance of global collaboration in tackling health crises. Moreover, there is an evident need to bolster data capacity at a global level, ensuring transparency and comprehensive data coverage, particularly in animal and environmental health sectors. Lastly, a more unified and consolidated governance structure for One Health is recommended, underscoring the urgency of translating One Health policies into actionable strategies across different levels of governance.