Carbonic anhydrase is an enzyme that catalyzes the rapid conversion of carbon dioxide and water to bicarbonate and protons, playing a pivotal role in CO 2 regulation and pH balance in biological systems. Its application in the cement and concrete industry is relatively novel, primarily focusing on enhancing the carbonation process to sequester carbon dioxide more effectively within cementitious materials. This biotechnological approach aims to improve the sustainability of concrete by reducing its carbon footprint through biological carbonation, which is particularly significant under the increasing pressures of global CO 2 emissions.