A dual nozzle vortex tube cooling system (VTCS) that delivers cooled compressed CO2 gas. This system aims to effectively lower the cutting temperature during the machining process of Ti-6Al-4 V. Experiments were conducted in this study with a constant cutting and flow parameters during turning at different levels of nozzle diameter. From the results, it was observed that decreasing the nozzle diameter reduced the cutting temperature and surface roughness and increased the cutting force. Furthermore, the optimization of the input parameters for better output response was done by means of the genetic algorithm. The experimental results were compared with the results obtained under different cooling media at the same machining conditions. There is a 37% and 66% minimizing the cutting temperature and surface roughness and a 14% increase in cutting force observed in CO2 VTCS compared to dry cutting.