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Detection methods used in food safety can be classified into two categories: amplification and hybridization. Their purpose is to accumulate or to recognize target sequence specifically using a proper reaction buffer in vitro. Polymerase chain reaction (PCR) is a valuable tool for monitoring gene expression, for quantifying foodborne pathogens, and for use in clinical diagnostics. Hybridization methods offer a new frontier in the identification of targets in a simple and high-throughput way. This review mainly describes and compares the basic rules of PCR and hybridization, including their formatting source, characteristics, and applications.

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Basic Rules in Nucleic Acid-Mediated Amplification and Hybridization Methods in Food Safety Detection: A Review

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  • 发表日期：

    2025年

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  • Wentao Xu

摘要

Detection methods used in food safety can be classified into two categories: amplification and hybridization. Their purpose is to accumulate or to recognize target sequence specifically using a proper reaction buffer in vitro. Polymerase chain reaction (PCR) is a valuable tool for monitoring gene expression, for quantifying foodborne pathogens, and for use in clinical diagnostics. Hybridization methods offer a new frontier in the identification of targets in a simple and high-throughput way. This review mainly describes and compares the basic rules of PCR and hybridization, including their formatting source, characteristics, and applications.