Lateral flow nucleic acid biosensors (LFNABs) provide a variety of advantages, including being affordable, sensitive, specific, user-friendly, rapid, equipment free, and deliverable to end users; thus, LFNABs have been very popular in biomedical, food, agriculture, environmental sciences, and so forth. This chapter discusses the latest progress on LFNABs, which primarily unite with a series of variants, such as constructions, signal systems, recognition systems, and amplification systems. Furthermore, we imagine LFNABs as an excellent candidate for available, fast, simple, and inexpensive detection with commendable applications.