Polyhydroxyalkanoates (PHAs) are internal microbial polyesters that are produced when there is an abundance of carbon sources and a scarcity of nutrients like nitrogen. They function as energy storage carbon reservoirs and provide prokaryotes with stress tolerance. These biopolymers are viable substitutes for carbon-based synthetic polymers (plastics) and have been previously isolated from bacteria growing on putrefied rice. The objective of this study was to isolate and characterize PHA and PHA-producing bacteria from the natural environment and to synthesize PHA-type biopolymer through a laboratory-scale procedure. Boiled and putrefied rice samples were utilized to collect, culture and isolate bacteria for the current study. These bacteria were then screened for PHA production capability. Sudan III dye staining was conducted to confirm the presence of PHA accumulation in the bacteria. When provided with a complex growth medium, bacteria growing in the medium exhibited diauxic growth. Molecular identification based-on 16srRNA marker gene confirmed the identity of the isolated PHA producing bacterium as Bacillus tropicus strain MCCC 1A01406. Polyhydroxyalkanoate produced from a cultured bacterial sample was characterized using Raman spectroscopy. Results from Raman analysis confirmed that the PHA produced was a copolymer between 3-hydroxybutyrate(3HB) and 3-hydroxyvalerate(3HV) units. Characteristic Raman markers confirm the presence of this copolymer which simultaneously occurred at the spectral positions 436 cm−1, 837 cm−1 and 1726 cm−1 in the Raman spectra. The diauxic growth together with the production of a copolymer confirms that the bacteria used in the present study were feeding on more than one carbon substrate. This study clearly demonstrated that free-living environmental organisms can be isolated and conveniently used for the purpose of polyhydroxybutyrate (PHB) bioplastics production.