The study investigates the development and refinement of edible films using tamarind seed starch (TSS), glycerol, and orange essential oil (OEO) as sustainable alternatives to traditional plastic packaging. Different formulations with different quantities of glycerol (3, 4, and 5 ml), OEO (1, 2, and 3 ml), and TSS (4, 5, and 6%) were investigated. After a thorough analysis, the ideal composition—which had favorable mechanical and physical properties—was found to be 5% TSS, 4% glycerol, and 2% OEO. The resulting films had thicknesses between 0.16 and 0.24 mm and had effective moisture barrier properties with water vapor transmission rates (WVTR) between 0.0012 and 0.0017 g/h·m2. Additionally, water solubility varied between 80.30 and 96.80%, indicating potential biodegradability, whereas tensile strength ranged from 0.01 to 0.1 MPa, suggesting moderate mechanical resilience. These results highlight the economic importance of using natural resources to design environmentally friendly packaging solutions, solving issues with plastic waste and promoting eco-friendly packaging industry practices.