Wounds always remain a primary medical concern and may be a result of various factors; some of them include traumatic injuries, surgical intervention, thermal burns, and even chronic conditions such as diabetes. In addition to posing significant management challenges for clinicians, wounds are often complicated by pathogenic infections that delay the healing process. Recent advancements in microbial nanomaterials in biomedicine promise to introduce a new wound treatment strategy. These materials have not only been specifically synthesised and designed in eco-friendly ways to satisfy wound healing needs but also improve our understanding of the wound microenvironment and its therapeutic potential in treating wounds. A diverse array of microorganisms has been employed to synthesize nanoparticles, and their applications associated with medical science have been successfully assessed. The number of microbially synthesized nanomaterials that facilitate wound healing is, however, still limited and scanty. In this present study, efforts are made to emphasise the available microbial nanoparticles; their progress is associated with wound healing treatment and analyses of existing therapies and their limitations. This chapter also points out the research gaps, suggesting areas for further investigation.