Burnout is an occupational condition of concern for health care workers, in particular nurses. Wearable sensors may be useful components of programs to monitor and improve burnout, but these devices should be usable. Prior experience with similar devices and length of exposure can influence perceptions about usability. We investigated the relationship between prior experience with and length of exposure to wristbands with perceived usability. Eighteen nurses from two hospitals wore the Empatica E4 wristband during working shifts for four weeks. Afterwards, they completed a survey including two yes/no questions regarding their experience and perceptions towards wristbands and the System Usability Scale (SUS). The Empatica E4 received an average SUS score of 85.14. Participants who wore wristbands before the study reported higher SUS scores, but participants who wore the Empatica E4 wristband longer in total, across more shifts and on shorter shifts reported lower SUS scores. Usability evaluations may more accurate when a longitudinal evaluation design is used.