Youth healthy lifestyle is undisputedly important as detrimental behaviours developed earlier in life could lead to many health risks during adulthood. The purpose of this research was to investigate differences in undergraduates’ healthy lifestyle behaviours according to gender. A total of 380 university students (male = 190; female = 190) from a private university in Kuala Lumpur, Malaysia were surveyed using the convenience sampling method. The Healthy Lifestyle Scale for Undergraduates Inventory was employed to examine undergraduates’ lifestyles. Data collected were analysed using descriptive and inferential statistics. Results revealed that both male and female students demonstrated three notable positive lifestyles for ‘health responsibility’, ‘life appreciation’, and ‘stress management’. However, both groups had minor impairment for the ‘health risk behaviour’. T-tests showed significant differences between males and females for ‘exercise behaviour’, ‘health risk behaviour’, and ‘social support’. Male students were more active but were more engaged in the ‘health risk behaviour’. Meanwhile, female students had better ‘social support behaviour’. The outcomes of this research could help relevant authorities develop effective strategies and programmes on campus to promote healthy lifestyle choices among this demographic.