In order to analyze the influence of soil anisotropy on slope stability analysis, an anisotropy yield criterion is introduced to evaluate the influence of varying shear strength on slope stability. The anisotropic characteristics of soil can be represented by the parameters n and β, which can be determined through experimental measurements. This paper constructs a complete finite element analysis framework for slope stability to accurately obtain the safety factor of slope (Fs). It is found that neglecting the anisotropy of soil will overestimate the stability of slope, especially when the slope angle (α) is small. The smaller the value of anisotropic parameter n is, the smaller Fs is. Fs will first decrease with the increase of anisotropic parameter β. When β increases to a critical value, Fs will increase with the increase of β. The greater the α is, the greater the critical value is.