Gram-negative bacterial infections pose a significant health challenge globally, particularly in wound care, where pathogens such as *Escherichia coli*, *Pseudomonas aeruginosa*, and *Acinetobacter baumannii* are prevalent. These infections are notorious for their resistance to conventional antibiotics and their ability to evade the immune response, leading to severe complications, including sepsis and high mortality rates. In India, these infections account for approximately 30% of hospital-acquired infections, highlighting the urgent need for innovative therapeutic strategies. This study investigates the efficacy of plasmonic photothermal therapy (PPTT) utilizing plasmonic nanoparticles (PNPs) to selectively target and eradicate Gram-negative bacteria. We demonstrate that PNPs, when exposed to near-infrared (NIR) laser irradiation, induce localized hyperthermia sufficient to destroy bacterial cells while sparing surrounding healthy tissue. Our results indicate that PNPs effectively bind to bacterial surfaces, promoting clustering and subsequent thermal destruction upon laser activation. In vitro assays reveal over 80% bacterial eradication within minutes of treatment without harming human red blood cells. This novel approach not only offers a viable alternative to traditional antibiotic therapies but also shows promise for enhancing wound healing outcomes, paving the way for future clinical trials and in vivo applications aimed at improving patient outcomes and reducing healthcare burdens associated with antibiotic resistance.