This research paper presents an experimental investigation conducted on Pulse Detonation Engine (PDE) utilizing acetylene as the fuel and oxygen as the oxidizer. The study focuses on the effects of multi-point fuel injection on the engine’s performance. The PDE test rig employed in the experiments featured a 48 mm inner diameter and a length of 1200 mm. Solenoid valves were utilized to control the intermittent supplies of acetylene and oxygen during the combustion process. The objective was to investigate the time-averaged thrust and specific impulse generated by the engine under various injection configurations to shortlist better performance parameters for a fixed form factor and allied conditions. The results obtained from the experiments revealed that the time-averaged thrust of the PDE test model was influenced by both the number of injectors and their respective locations. For a detonation frequency of 10 Hz, the time-averaged thrust ranged between 200 and 500 N, depending on the specific injector configuration used. These findings shed light on the significance of optimizing the injection parameters, including the number and placement of injectors, to achieve desired performance characteristics in PDEs. The experimental investigation contributes to the understanding of multi-point fuel injection and its impact on the overall efficiency and thrust generation capabilities of pulse detonation engines, which will play detrimental role in realizing flight-worthy hardware.