Modern agriculture is currently facing significant challenges, including climate change, human population growth, increased food demand, reduced agricultural land, and environmental pollution. Precision agriculture has emerged as a vital strategy to optimize resource use, enhance productivity, and decrease environmental impact. In this context, enzymatic nanobiosensors represent an innovative technology with the potential to transform agricultural practices. These devices combine principles of nanotechnology (NT) and molecular biology to accurately and in real time detect various chemical and biological variables, such as nutrients, contaminants, and pathogens. This capability enables more informed and efficient decisions in crop management. The use of advanced sensors in agriculture is essential for addressing global challenges like climate change, water scarcity, and rising food demand. However, traditional monitoring techniques tend to be slow and costly and often require specialized personnel. In contrast, enzymatic nanobiosensors offer high sensitivity and selectivity and can be integrated into smart systems, making them particularly suitable for precision agriculture. These sensors can detect specific levels of key compounds, such as nitrates and phosphates, optimizing fertilization while minimizing environmental impact. Enzymatic nanobiosensors can significantly contribute to precision agriculture by developing next-generation diagnostic tools and techniques. This chapter aims to explore the design, fabrication, and application methods of enzymatic nanobiosensors in precision agriculture, focusing on their ability to effectively detect nutrients and contaminants. It also seeks to identify the primary technological and environmental limitations hindering their widespread adoption and propose strategies for their implementation in smart agricultural systems.