Drive cycle (DC) is a speed vs time relation, which represents localised data of driving pattern which is mostly depends on local traffic, individual behaviour, type of highway, geometry of highway and other factors such as seasonal variation and environmental factors. DCs are used for estimation of mechanical requirements, Mileage and emission in two-, three- and four-wheeler vehicles. This paper mainly focused on derivation of power requirement, total tractive effort (TTE) and torque requirement from real-world two-wheeler DC and comparing the output to synthetic DC, i.e. MoRTH DC. Each output is derived by developing a MATLAB/SIMULINK model for each DC. The results indicates that there is a significant variation in TTE, power requirement and torque between real-world DC from four cities and synthetic DC. MoRTH DC which has lowest parametric and output values when comparing them with real-world DC from other mentioned cities. Power required for Hyderabad DC was 288.19%, torque requirement was 448.84%, and total tractive effort required was 622.7% more than MoRTH DC output. Hence, for two-wheeler designing and testing, it is concluded that real-world DC gives us more practical and realistic output. Thus, real-world DC should also be used to measure other parameters, such as emissions, which are often underestimated when using synthetic DC. Therefore, to effectively limit emissions and formulate benchmark emission standards for policy frameworks, adopting real-world DC is the way forward.