The cardiovascular disease is a common disorder and is considered as one of the leading causes of death around the world. The early detection of cardiovascular diseases is urgently needed. Though some diagnostic biomarkers have been used in prediction of diverse cardiovascular diseases in clinical practice, they are not enough good as expected. The urine proteome studies of cardiovascular diseases were relatively less than the plasma studies, however, they may provide some useful clues for prediction of cardiovascular diseases with the development of high-resolution mass spectrometry technology. In this chapter, we summarized the urinary diagnostic protein biomarker of cardiovascular diseases (animal models and patients) and reviewed recent urine proteome studies of coronary artery disease, myocarditis, and heart failure. These findings may provide clues for the pathogenesis of diseases and the results may show that urine is a potential source of cardiovascular disease biomarkers.