This research investigates wastewater by-product treatment of biosolids’ sludge, and the environmental performance of desludging projects in regional Western Australia (WA). This work quantifies the environmental impacts of desludging; a life-cycle assessment (LCA) technique is used to examine global warming potential, eutrophication, and ozone-layer depletion potential, over a specified system boundary, at three wastewater-desludging treatment plants by, calculating the outputs for the key impact-categories during facility construction, operation and the end-of-life phases at the case-study WA locations of Cape Burney, Kalbarri, and Fitzroy Crossing. This work concludes that the greatest localised (Western Australian) environmental impact is attributed to eutrophication, followed by global-warming potential. Ozone-layer depletion had negligible impact on environmental impact assessment upon these case-study locations in regional WA. The highest emission output is associated with removal of sludge material from operational-phase ponds. Whilst a preferred desludging approach was not found due to respective transportation distances’ disparities, the research here does provide a solid preliminary understanding of the environmental impacts of desludging processes of waste stabilisation ponds in WA, and the importance of applying appropriate (LCA-directed) decision-making criteria for the selection of site-specific desludging approaches. These findings assist future policymaking for desludging projects in regional WA, towards minimising regional environment impact.