Laser surface remelting under the influence of reactive gas to develop nitride over the nitride titanium sample is a widely used method. The presented study aims to perform in-situ laser remelting of additively manufactured Ti6Al4V substrate under the nitrogen environment in order to generate nitride over the surface for the use in biomedical industry. In order to achieve this a reactive environment of nitrogen (N) is introduced in the laser additive build chamber for laser remelting of additively pre-build Ti6Al4V substrate. Surface topography and elemental changes with remelting were studied to analyse the possible state of the remelted surface. Results show an increase in surface roughness with selective laser remelting, which could enhance cell adhesion and Osseointegration, which is advantageous for biomedical purposes. The EDX analysis shows an increase in the atomic weight percentage of nitrogen. X-ray Photon Electron Spectrometry (XPS) also indicates the formation of nitrogen bonds with Ti and Al, which were initially missing in substrates. The finding suggests the in-situ remelting under the nitrogen environment modifies the surface properties of additively manufactured Ti6Al4V parts which could be possibly useful for the biomedical industry.