Scholarlink

Caenorhabditis elegans, a small soil-dwelling nematode, has become one of the most valuable and widely used model organisms in biological and genomic research since its introduction into the laboratory over 50 years ago. Its appeal arises from its ease of maintenance, minimal nutritional requirements, rapid reproduction and long history of scientific study. C. elegans has the unique ability to self-fertilize and produce many offspring in a short period of time, with a life cycle of about 3 days at 25 °C. It was the first multicellular organism to have its genome fully sequenced, underpinning its importance in genomics. C. elegans has proven to be particularly useful for studies in genetics, cell biology, neurobiology and aging, thanks to its well-characterized genome and its transparent body, which allows non-invasive observation of internal structures. C. elegans has a simple but functional anatomy with a mouth, pharynx, intestine and gonads, with roughly 1000 somatic cells in the adult animal. Hermaphrodites and males differ in their sex chromosomes. Hermaphrodites have two X chromosomes, while males have only one X chromosome (XO). Their rapid growth, predictable developmental stages, and large offspring make them an ideal model for high-throughput research, including studies of aging, disease models, and drug testing (Stiernagle, WormBook. https://doi.org/10.1895/wormbook.1.101.1, 2006). This chapter provides a comprehensive guide to basic and essential protocols for handling C. elegans, making it a valuable resource for new and experienced researchers. Key techniques covered include media preparation, methods for synchronizing C. elegans cultures after embryo isolation, infection models, and microscopy techniques for observing nematode development. The chapter also provides guidelines for freezing and long-term preservation of strains. This chapter emphasizes the utility of C. elegans as a versatile and powerful organism in numerous areas of biological research. It provides a basic understanding of the use of this model in laboratory settings.

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

In Vivo Assay Using Caenorhabditis elegans

  • 章节

  • 发表日期：

    2025年

查看全文
  • Ritu Pasrija,
  • Deepika Kumari

摘要

Caenorhabditis elegans, a small soil-dwelling nematode, has become one of the most valuable and widely used model organisms in biological and genomic research since its introduction into the laboratory over 50 years ago. Its appeal arises from its ease of maintenance, minimal nutritional requirements, rapid reproduction and long history of scientific study. C. elegans has the unique ability to self-fertilize and produce many offspring in a short period of time, with a life cycle of about 3 days at 25 °C. It was the first multicellular organism to have its genome fully sequenced, underpinning its importance in genomics. C. elegans has proven to be particularly useful for studies in genetics, cell biology, neurobiology and aging, thanks to its well-characterized genome and its transparent body, which allows non-invasive observation of internal structures. C. elegans has a simple but functional anatomy with a mouth, pharynx, intestine and gonads, with roughly 1000 somatic cells in the adult animal. Hermaphrodites and males differ in their sex chromosomes. Hermaphrodites have two X chromosomes, while males have only one X chromosome (XO). Their rapid growth, predictable developmental stages, and large offspring make them an ideal model for high-throughput research, including studies of aging, disease models, and drug testing (Stiernagle, WormBook. https://doi.org/10.1895/wormbook.1.101.1, 2006). This chapter provides a comprehensive guide to basic and essential protocols for handling C. elegans, making it a valuable resource for new and experienced researchers. Key techniques covered include media preparation, methods for synchronizing C. elegans cultures after embryo isolation, infection models, and microscopy techniques for observing nematode development. The chapter also provides guidelines for freezing and long-term preservation of strains. This chapter emphasizes the utility of C. elegans as a versatile and powerful organism in numerous areas of biological research. It provides a basic understanding of the use of this model in laboratory settings.