Microplastic pollution in soil is an emerging environmental concern with widespread implications for ecosystem health and human well-being. Originating from various sources such as agriculture, industrial activities, and atmospheric fallout, microplastics (MPs) persist in soil environment, altering soil chemistry, physical properties, and biological interactions. These minute plastic particles, predominantly composed of polyethylene (PE) and polystyrene (PS), accumulate in soil as various shapes like films, spheres, beads, and fragments posing risks to soil biota and potentially entering the food chain. Current research highlights the need for standardized methods in sampling, pre-treatment, and analysis to accurately quantify and characterize MPs in soil. Techniques such as extraction of MPs from the soil, including digestion of organic matter, density separation, and chemical characterization with spectroscopic analysis (e.g., Fourier Transform Infrared spectroscopy (FTIR), Raman spectroscopy), are essential in enhancing scientific understanding of MPs pollution in soil. Studies indicate that MPs can disrupt soil nutrient cycling, microbial diversity, and plant growth, which affect agricultural productivity and ecosystem stability. Despite increasing awareness, there are gaps in understanding the long-term impacts of MPs on soil health and biodiversity. Addressing this issue requires collaborative efforts in monitoring, implementing mitigation strategies, and conducting additional research to formulate sustainable solutions. Establishing standardized protocols for assessing MPs in soil is important for comprehensive risk evaluation and efficient environmental management.