The rise of antibiotic resistance (AR) bacteria poses a serious threat to the global healthcare systems and urgently needs the development of rapid and sensitive diagnostic methods for effective treatment. Nanoscale sensors, utilizing advanced nanomaterials and cutting-edge bio-recognition elements, offer a promising solution for the development of point-of-care (POC) diagnostic devices. The POC devices are crucial tool for timely diagnosis and management of diseases, particularly in low resource settings, where conventional laboratory-based methods are impractical or commercially not viable. Here, we have discussed the molecular mechanism of AR, molecular and biochemical basis of AR, key biomarkers associated with AR, and current status of commercially viable and field-deployable sensor prototypes. Advanced field-deployable techniques, such as paper-based tests, microfluidic systems, LAMP, and CRISPR-based assays, are discussed for their advantages over conventional methods. The prototypes that have the potential for mass production and further commercialization, and their working principle/mechanism of AR detection are prioritized here.