ZnO, a multifunctional material has viability for various applications in a specific nanostructured form like four legged tetrapods. In this work, ZnO tetrapods synthesized by a modified catalyst free thermal evaporation technique is attempted to test for plausible self-assembly under gravity in a homogenous toluene medium. Based on the theoretical modeling and analysis on the self-assembly of the coarse-grained bead-spring model of tetrapods, preliminary control experiments were designed and attempted using FESEM image analysis. These control experiments were carried out by taking 3 ml of toluene as a homogenous and moderately viscous medium. 10 mg of ZnO tetrapods was taken in three beakers with toluene and is ultrasonicated. Three different substrates have been considered for assembling the tetrapods on their surface including carbon tape, copper sheet and Indium Tin Oxide coated glass (ITO). All the substrates were placed horizontally into separate beakers and the ultrasonicated ZnO tetrapods were allowed to settle over them. After complete evaporation of toluene from the beakers, the substrates were carefully taken for image analysis. The FESEM images confirmed the settlement of the ZnO tetrapods. However, the number density of tetrapods standing upright was very negligible. Although the experimental results confirmed the formation of small number of interlocked structures and vertically aligned tetrapods, the experimental data deviated from the self-assembly structures from those predicted by theoretical simulations. These control experiments need to be further tuned to extend statistical properties such as ensemble average mean square displacement, diffusion constant, etc.