This chapter provides an introductory overview of bioinformatics, a rapidly evolving field that has become increasingly relevant in biological research. It briefly discusses the historical influence of the Human Genome Project and provides a basic understanding of bioinformatics concepts without delving into advanced techniques or specialized methodologies. The chapter highlights the growth of biological data, particularly driven by next-generation sequencing technologies, and the resulting need for sophisticated computational tools to process and analyze these data. While AI is expected to contribute significantly to the future development of bioinformatics, this chapter does not focus on cutting-edge technologies. Instead, it aims to introduce readers to the foundational principles and essential tools of bioinformatics. While the chapter touches on bioinformatics applications in areas such as genomics and viroinformatics, it does not go into detail. Its primary purpose is to provide readers with a solid grounding in core bioinformatics concepts, helping them understand its scope and significance in biological research. By offering this basic introduction, the chapter prepares readers for a deeper exploration of bioinformatics in later sections, where more advanced methodologies and applications are discussed. This chapter is intended as a stepping stone for those new to the field, providing the knowledge essential to build a strong foundation in bioinformatics.