Laser grooving is the process to create a groove on the workpiece surface using laser in a minimum possible time with minimal harm for the purpose of application in microfluidic and heat transfer in electronics and biomedical devices. The paper focus on the generation of better quality groove using fiber laser which finds application in many organization because of its compactness, flexibility, and less maintenance. Experimental results revealed that better grooving characteristics on the surface of workpiece was observed at a laser irradiation of 45% of 50 W, pulse rate of 120 kHz and scan rate of 35 mm/s.