As compared to cancer cells to their normal counterparts explain distinctive biomechanical and material properties that are essential to interpreting the behaviour, course, and responsiveness of cancer cells to medication. Cytoskeleton and extracellular matrix modification impact the distinct mechanical properties of cancerous cells, with enhanced deformability, changed viscoelasticity, and changing adhesion property. Changes affect the total hallmarks of cancer. Furthermore, cancer cells’ mechanical characteristics can be useful for therapeutic and diagnostic purpose. In order to know the mechanobiology of cancerous cells, many techniques help to understand their biomechanical features. These techniques are optical tweezers, microfluidics, and atomic force microscopy (AFM). The innovation of diagnostic techniques which differentiate cancerous cells from normal cells by using mechanical process can be facilitated by knowing these characteristics. Furthermore, novel therapeutic approaches can be attainable if therapies work on the biomechanical characteristics of cancerous cells, like those that affect adhesion and stiffness. Here, possible uses in cancer detection and treatment, biomechanics and material characteristics play a crucial part in the biology of cancer.