The ability to determine the mechanical characterizations of bone at microscopic level has a great importance in biological and clinical application. Fatigue and static fracture generally occur due to repeated load or low intensity load for long term rather than trauma, most of the cases of fatigue failure is found in military, athletes, ballet dancer and in young children and frequently increases. The purpose of present study is to examine the mechanical properties of hydrated and non-hydrated bovine compact bone using nanoindentation technique. Here we examine elastic modulus, hardness, compressive and tensile strength and their viscoelastic nature by measuring storage and loss modulus of both grouped bones (Hydrated and non-hydrated).