Doping of quantum dots (QDs) in liquid crystal (LC) is a burgeoning area of research since it utilizes the properties of both materials and combines them together for their mutual benefits. Unique electronic and optical characteristics of QDs influence the inherent characteristics of LC materials which involves optimization of their compatibility and interactions to achieve a desired functionality in the final composite. Herein, we report the effect of doping of MoS2 QDs on the dielectric and optical characteristics of a homogenously aligned nematic LC, namely 4-cyano-4′-pentylbiphenyl (5CB) using crossed polarized microscope and dielectric spectroscopy. Optical micrographs revealed that increasing concentration of MoS2 QDs shows the formation of aggregated QDs in homogeneously aligned NLC. However, the alignment of composite remains similar to that of Pure 5CB. Frequency and concentration-dependent variation in the perpendicular (i.e., transverse) component of dielectric permittivity (εʹ⊥) for Pure 5CB and MoS2-5CB composites shows that in 5μL of MoS2 QDs, εʹ⊥ decreased, and in 10μL, it showed an increment with respect to Pure 5CB. An external bias field of 17 V is also applied to observe the change in longitudinal component of dielectric permittivity (εʹ||) and short axis relaxation (spectral peak and amplitude) for Pure 5CB and MoS2-5CB composites. Moreover, electrical conductivity of 5CB was found to increase by 3.6 and 4.9 times in 5μL and 10μL MoS2 QDs-5CB composites, respectively. We do anticipate that such composites would have potential for their application in high-performance LC displays and other non-display devices.