Metabolomic profiling, an advanced tool used to identify different metabolite biomarkers after exposure to different substances, has high sensitivity and reproducibility. This method can detect sensitive changes in biological metabolites under several conditions; thus, it is used to examine the effects of vaping on human health. The main concern with vapes is the potential respiratory issues caused by the detrimental components in their emissions. The new findings not only challenge recognized assumptions but also carry substantial health risks for individuals exposed to them. Metals such as aluminum (Al), antimony (Sb), chromium (Cr), copper (Cu), and lead (Pb) are present in e-liquids according to metabolomics at levels higher than those previously thought in past studies. The highest levels of formaldehyde found in electronic cigarette (e-cig) aerosols due to occupational exposure are far from the safety limits; thus, biological systems should be able to monitor these changes. When nicotine-containing aerosols are inhaled, second-hand exposure occurs. The presence of nicotine-containing aerosols results in second-hand exposure, with flavored liquids, particularly cherry, showing higher levels of benzaldehyde. Furthermore, e-cig emissions contribute to air pollution through the release of particulate matter, but evidence of their overall impact is conflicting; therefore, additional metabolomics studies aimed at elucidating this issue are needed. The need for continuous research on e-cigs cannot be ignored given our limited knowledge about long-term effects coupled with similarities between toxicity mechanisms exhibited by both conventional cigarettes and electronic ones. Therefore, metabolomic tools should be effectively utilized during such investigations. Regulations for vapes vary from one country to another; hence, strict supervision is needed globally while enhancing community awareness and putting in place preventive measures to minimize the risks associated with their usage.