We report on a series of experiments examining the effects of the number of shock wave exposure on drug delivery and cell viability. Drug internalization and cell viability experiments are performed on human embryonic kidney cells (HEK293) using fluorescently-tagged molecules (FD10) with flow cytometry and trypan blue exclusion assay. It is observed that initially increasing shock wave exposure leads to increased FD10 internalization with high cell viability. It is found that increasing the number of shock waves from these conditions does not effectively increase drug internalization but decreases cell viability. It is also found that the number of shock wave exposures did not affect the proliferation rate of treated cells that survived the treatment, even for a large number of shock wave exposures. From these results, we are investigating conditions that may increase internalization with minimal shock wave exposure while maintaining high cell viability.