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Tilapia Lake Virus (TiLV) and Tilapia Parvovirus (TiPV) are two emerging pathogens causing major threats to the global tilapia aquaculture industry. This chapter covers the applications of RT-qPCR as a highly sensitive and specific, useful diagnostic tool in detecting and quantifying these viruses. The introduction exemplifies the impact of the presence of TiLV and TiPV on aquaculture and emphasizes the necessity for accurate diagnostic tools in the effective management of diseases. Real-time RT-PCR (PCR) is best known for its high sensitivity and specificity, rapid results, scalability, lower potential for contamination or errors, and its inherently quantitative nature. It has been used in many laboratories to detect and monitor the Tilapia Lake Virus (TiLV) and Tilapia ParvoVirus (TiPV), two fatal diseases affecting tilapia farms worldwide. In this chapter, we describe the procedure of sample collection, DNA extraction, RNA extraction, reverse transcriptase-PCR for cDNA synthesis, and rRT-PCR assay for detecting and quantifying the diseases TiLV and TiPV.

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Detection and Quantification of Tilapia Lake Virus (TiLV) and Tilapia Parvovirus (TiPV) by Real-Time PCR

  • 章节

  • 发表日期：

    2025年

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  • Rahul Krishnan,
  • Devika Pillai

摘要

Tilapia Lake Virus (TiLV) and Tilapia Parvovirus (TiPV) are two emerging pathogens causing major threats to the global tilapia aquaculture industry. This chapter covers the applications of RT-qPCR as a highly sensitive and specific, useful diagnostic tool in detecting and quantifying these viruses. The introduction exemplifies the impact of the presence of TiLV and TiPV on aquaculture and emphasizes the necessity for accurate diagnostic tools in the effective management of diseases. Real-time RT-PCR (PCR) is best known for its high sensitivity and specificity, rapid results, scalability, lower potential for contamination or errors, and its inherently quantitative nature. It has been used in many laboratories to detect and monitor the Tilapia Lake Virus (TiLV) and Tilapia ParvoVirus (TiPV), two fatal diseases affecting tilapia farms worldwide. In this chapter, we describe the procedure of sample collection, DNA extraction, RNA extraction, reverse transcriptase-PCR for cDNA synthesis, and rRT-PCR assay for detecting and quantifying the diseases TiLV and TiPV.