Photochromic materials can exhibit reversible color changes under illumination within specific wavelength ranges and be applied in many fields, such as anti-counterfeiting, decoration and military. This research used the photochromic compound spiropyrans as the core material, hexadecane as the solvent, and urea-formaldehyde resin as the shell material. Photochromic microcapsules were prepared through an in-situ polymerization method. The structure, morphology, and thermal stability of the microcapsules were characterized by using Fourier Transform infrared spectroscopy (FTIR), Confocal laser scanning microscope (CLSM), and Thermogravimetric Analysis (TG). The average particle size of the prepared microcapsules is about 30 μ m. The microcapsules also show good fatigue resistance when dissolved in water. Photochromic microcapsule ink for screen printing is prepared by mixing the microcapsules with transparent paste and polyvinyl alcohol resin (PVA) in specific proportions. Meanwhile, the photochromic performance was examined using a portable spectrophotometer to test the color parameters of samples before and after color change. The results indicate that the photochromic microcapsules prepared by the abovementioned method possess good photochromic properties at room temperature. Color-changing products with anti-counterfeiting and fatigue resistance features can be obtained using a screen printing process to transfer the photochromic ink onto different flexible substrates.