Discomfort among poultry farm workers in northeast India was studied in three operations, namely feeding, cleaning, and catching hens. Data were collected using a questionnaire survey among 120 workers in the age group of 17–68 years. Discomfort levels on nine body parts, such as upper back, neck, shoulder, arms, wrists, legs, lower back, feet, and hips were considered in the survey. The collected data were analysed to study the effect of gender, experience, and rearing methods on the level of discomfort. The females have significantly higher (p < 0.05) discomfort in the lower back, shoulder, and arms in all activities than the males. Workers with more than 10 years’ experience reported significantly (p < 0.05) higher discomfort in the shoulder during feeding and catching hens compared with less than ten years of experience. The ground-rearing method has significantly (p < 0.05) higher discomfort in the arms than the change method.