Buildings negatively impact species wellbeing by contributing to land use and biodiversity loss. Assessing these impacts is complex and requires a synergistic approach to environmental assessment, ecological indicators and lifecycle thinking. This study aims to develop a scoping approach for ecological influences for the biodiversity lifecycle impact assessment of the built environment. We adapt and combine the Biodiversity Net Gain (BNG), Ecological Impact Assessment (EcIA) and ReCiPe Lifecycle Impact Assessment methods to the Australian context. The combined method involves (1) obtaining the input data for species, land and ecology; (2) defining the characteristics, values and conditions of biodiversity, land cover, habitat and ecosystem; (3) assigning ecological and habitat scores to these characteristics, and (4) determining the biodiversity, land use and habitat impact assessment outputs. We demonstrate this approach using selected areas of Greater Sydney as a case study. Findings inform a framework to define and collect data for a biodiversity lifecycle impact assessment of a building. The outcomes help develop a biodiversity assessment method for buildings and expand ecological values mapping of the natural environment to include the built environment. Implementation of the study findings could help to minimise species loss in urban environments.